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Fig. 3 | Microbiome

Fig. 3

From: Strain-level resolution and pneumococcal carriage dynamics by single-molecule real-time (SMRT) sequencing of the plyNCR marker: a longitudinal study in Swiss infants

Fig. 3

Flowchart of nasal swab (NS) sample processing for PacBio SMRT sequencing. Molecular processing of NS samples for plyNCR sequencing (strain identification) highlighted in peach. 1PCR performed using primers with PacBio universal sequence overhang. *QC: quality control excludes amplicons with no observable peak or multiple peaks on the fragment analyzer. VGS, viridans group streptococci

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Microbiome

ISSN: 2049-2618

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