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Table 3 Changes in genes and transcripts (copies per million, CPM) encoding for methyl-coenzyme M reductase (MCR) enzyme (EC: 2.8.4.1) in the rumen of dairy cows supplemented with 3-nitrooxypropanol (3-NOP) compared to control cows at weeks 4, 8, and 12. Based on raw reads and based on metagenome assembled genomes (MAGs). K00399, alpha subunit of MCR; K00401, beta subunit of MCR; K00402, gamma subunit of MCR

From: The effect of 3-nitrooxypropanol, a potent methane inhibitor, on ruminal microbial gene expression profiles in dairy cows

MCR genes identification based on raw reads
  Metagenomics
W4 W8 W12  
  Control 3-NOP % reduction Control 3-NOP % reduction Control 3-NOP % reduction Treatment P value
K00399 265 213 20 306 230 25 227 253 −11 0.18
K00401 245 178 27 239 163 32 169 192 −14 0.18
K00402 253 197 22 293 223 24 210 251 −20 0.22
2.8.4.1 763 588 23 838 616 26 606 697 −15 0.12
Metatranscriptomics
K00399 2756 2578 6 3656 2548 30 2667 2333 13 0.15
K00401 2135 1781 17 2529 1606 36 1885 1415 25 0.001
K00402 2679 2908 −9 3946 2905 26 2829 2571 9 0.64
EC:2.8.4.1 7570 7268 4 10131 7059 30 7381 6320 14 0.06
MCR gene identification based on MAGs
Metagenomics
K00399 32 33 −3 49 47 5 36 51 −42 0.47
K00401 52 43 17 55 37 33 38 42 −11 0.10
K00402 142 92 35 88 41 54 69 54 22 0.06
EC:2.8.4.1 75 56 26 64 42 35 48 49 −3 0.07
Metatranscriptomics
K00399 411 507 −24 777 607 22 596 591 1 0.92
K00401 537 565 −5 877 570 35 681 545 20 0.08
K00402 1282 988 23 1276 816 36 891 605 32 0.02
EC:2.8.4.1 743 687 8 977 664 32 723 580 20 0.01
  1. \(\mathrm{Percent}\ \mathrm{reduction}=\frac{\left(\mathrm{Control}\right)-\left(3-\mathrm{NOP}\right)}{\left(\mathrm{Control}\right)}\ \mathrm{X}\ 100\)