Fig. 2

Phylogenetic tree of MAGs obtained from SHIPPO. a A phylogenetic tree of prokaryotic SHIPPO MAGs using core genes from Phylophlan2. A total of 252 MAGs were obtained; each bar outside the tree represents the number of methyltransferase (MTase) genes present in each MAG. b The distribution of restriction enzyme and MTase types from SHIPPO MAGs across kingdoms. c Prokaryotic SHIPPO MAGs were compared against genomes from Tara Oceans (TARA) and Global Ocean Reference Genomes Tropics (GORG-Tropics) datasets using FastANI. d The number of genes associated with the restriction-modification (RM) system is plotted against the genome size for each ocean microbiome MAG (SHIPPO MAG, TARA, and GORG-Tropics). Points are shaped depending on the type of the complete and orphan RM system. MAG: metagenome-assembled genome; SHIPPO: Shipborne Pole-to-Pole Observations