Fig. 2
From: Location-specific signatures of Crohn’s disease at a multi-omics scale
Multi-omics identifies features both common and unique features of ileal- and colonic-isolated diseases. A Enrichr-generated gene ontology enrichment graph using host proteome features significantly greater in CCD compared to ICD, sorted by Enrichr combination score [23]. B Comparison of neutrophil-associated proteins and SES-CD Spearman correlation scores, binned by CD location. Significance derived using Welch’s t test, *** = p < 0.0001. n = 49 features, all active disease ICD (n = 31) and CCD (n = 24) subjects used in comparison. C Heatmap of neutrophil-associated protein abundance (x-axis) compared in healthy controls, CD subtypes, and UC. Abundance is column scaled to enhance visibility. D Bar plot comparing log2-transformed relative protein abundance between ICD (n = 31) and CCD (n = 24). *p < 0.05, ***p < 0.0001, Welch’s t test. E Comparison of dipeptide/amino acid abundances between ICD (n = 31) and CCD (n = 24). Mean abundance levels plotted. Each dot represents a different amino acid/dipeptide identified and lines connecting dots denote the same metabolite in the disease location specified. F Comparison of blood-related proteins (complement C4, fibrinogen, and serum albumin) found in ICD (n = 31) and CCD (n = 24) stool samples. **** = p < 0.0001, Welch’s t test. G PCA biplot of individual CD locations, overlayed with SES-CD severity scores. Color ramping from purple to yellow denotes increasing severity score. Only five most contributing features (combined PCA1 + 2 contribution) plotted for ease of viewing. H Scatter plot comparing SES-CD scores (x-axis) and B. vulgatus metagenomic abundance. Linear model used to plot trend line. ICD n = 31, Rho = 0.29, p = 0.16; CCD n = 24, Rho = 0.48, p = 0.03; ICCD n = 11, Rho = 0.84, p = 0.07. I Bar plot comparing Spearman correlation values (n = 89 corr. pairs) of B. vulgatus levels and neutrophil related proteins, split by ICD and CCD. ****p < 0.0001, Welch’s t test. J Bar plot comparing significant (p < 0.05) Spearman correlation values of B. vulgatus levels and dipeptide metabolomic features, split by ICD (n = 6 features), and CCD (n = 31 features). ****p < 0.0001, Welch’s t test