Table 1 Configurations of the simulation settings used in the evaluation of differential abundant analysis methods
Setting | Sample size (n) | Taxa number (m) | Depth confounding | Signal density | Differential mode | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
50 | 100 | 200 | 50 | 500 | No | Yes (-fold) | Low | Medium | High | Abundant | Rare | |||||
4 | 9 | 5% | 10% | 20% | ||||||||||||
Binary | Global null | 1 | ✓ | ✓ | ✓ | ✓ | ✓ | |||||||||
Balanced | Basic setting | 2 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ||||||
Small n | 3 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ||||||
Small m | 4 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | |||||||
Depth confounding | 5 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ||||||
Unbalanced | Basic setting | 6 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | |||||||
Small n | 7 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ||||||||
Small m | 8 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | |||||||
Binary + confounder | Balanced | Basic setting | 9 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | |||||
Unbalanced | Basic setting | 10 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | |||||||