Fig. 2

Sampling time point is associated with significant differences in fecal resistome and microbiome composition. A Dendrogram showing linkage clustering of Bray-Curtis (BC) dis(similarities) of fecal resistome composition at the ARG level, colored by time point and treatment group. Non-metric multidimensional scaling (NMDS) ordination based on BC (dis)similarities (stress=0.076) by B time point (ANOSIM—analysis of similarities P=0.001, PERMANOVA—permutational multivariate analysis of variance R²=53.9%, P=0.001) and C treatment group (ANOSIM P=0.07, PERMANOVA R²<1%, P=0.22). NMDS ordination based on BC (dis)similarities (stress=0.10) of microbial genera by D time point (ANOSIM P=0.001, PERMANOVA R²=42.6%, P=0.001) and E treatment group (ANOSIM, P=0.32, PERMANOVA R²<1%, P=0.11). Ellipses indicate the 95% confidence interval for distance from the centroids of each respective group of samples