Fig. 4
From: Community structure of coral microbiomes is dependent on host morphology
nifH diversity across coral species. A Average relative abundances of nifH phylotypes (unique sequence variants) belonging to nitrogen-fixing clusters I and III. Bar segments are colored according to placement in B phylogeny of nifH amplicons. Peptide translations for de novo nifH and best-match NCBI nifH sequences were aligned with MAFFT along with nitrogenase-like chlorophyllide reductases. De novo nifH that placed within the chlorophyllide/ferrodoxin clades were excluded. Only nifH phylotypes with phylogenetic relationships to established nitrogen-fixing nifH clades (e.g., clusters I and III) were subsequently analyzed. Taxonomic affinity of nifH variants is based on phylogenetic placement using published sequences. Note: only phylotypes representing at least 1% of all reads were included