Fig. 1From: Quantitative microbial population study reveals geographical differences in bacterial symbionts of Ixodes ricinusSampling sites of I. ricinus in the Netherlands. Pooled and individual ticks from six forest sites (triangles) were used for a 16s rRNA amplicon 171 sequencing analysis. Individual nymphs from these and 13 (points) other forest sites were tested by 172 qPCR for the presence of tick symbionts. A box marks the sampling site by two letters, and a linear 173 colour gradient represents latitude-longitude. Full coordinates, habitat, vegetation cover, tick 174 densities, and a number of vertebrate species per locations are provided in Additional file 1: Table S1Back to article page