Fig. 5

Differences in virome composition of UC-HMA mice after VLP gavage. Data shown is from experiment B (trial #1). A NMDS of Bray-Curtis dissimilarity of scaffolds between HMA mice given healthy VLPs, UC VLPs, or PBS during the (left) bacterial colonization period, (middle) VLP gavage period, or (right) DSS/washout period. Significant differences in Bray-Curtis dissimilarity were assessed in each time period using adonis PERMANOVA (p ≤ 0.05). Dots represent pooled mouse fecal samples at a single sampling point. All sampling points of the longitudinal study were included in the NMDS and comparative analyses. NMDS stress: bacterial colonization period (stress = 0.146), VLP gavage period (stress = 0.181), DSS/washout (stress = 0.148). B Relative abundance of viral families between treatment groups over the experimental time periods. (C) DESeq2 differentially abundant scaffolds between UC-HMA mice given healthy (left) or UC (right) VLPs compared to the PBS control. Differentially abundant scaffolds represent those that significantly changed after VLP gavage between treatment groups. Scaffolds with adjusted p values ≤ 0.01 and with log₂fold changes greater or less than 1 were considered differentially abundant using a two-tailed wald test. Black points on volcano plots indicate overlayed under-abundant scaffolds. At each sampling point, mouse fecal samples in each cage were pooled from 2 mice (n=3 cages per group, 6 mice per group; UCbac, UC-HMA mice)