Fig. 3

Fungi do not seem to have an effect on double Dectin-deficient mice with DSS-induced colitis. A, B Dectin-1/2-deficient (D-1/2KO) mice received C. tropicalis (C. trop) or M. restricta (M. rest) and then dextran sulfate sodium (DSS) for 7 days. PBS n = 7, C. trop n = 6, M. rest n = 7. A Experimental design for the administration of fungi and DSS. B Weight of DSS-exposed mice (left panel), disease activity index (DAI) of DSS-exposed mice (middle panel) and length of the colons of mice treated with DSS (right panel). C, D D-1/2KO mice received fluconazole (Fluco) and then DSS for 7 days. PBS n = 3, Fluco n = 8. C Experimental design for the administration of an antifungal agent and DSS. D Weight of DSS-exposed mice (left panel), disease activity index (DAI) of DSS-exposed mice (middle panel) and length of the colons of mice treated with DSS (right panel). E, F Wild-type mice from Janvier Laboratory (WTj) and Dectin-1/2 deficient (D-1/2KO) mice without challenge. WTj n = 14, D-1/2KO n = 13. E Shannon index describing the alpha diversity of the fungal microbiota (ITS) in the fecal microbiota. F Beta diversity. Principal coordinate analysis of Jaccard distance, with each sample colored according to the genotype