Fig. 1

Dectin-deficient mice react differently to DSS-induced colitis. A ELISA of the expression of IL-6 (left panel) and TNFα (right panel) in BMDC cultures from wild-type (WT), Dectin-1-deficient (D-1KO), Dectin-2-deficient (D-2KO), and Dectin-1/2-deficient (D-1/2KO) mice stimulated with fungal ligands (zymosan and mannan). B–F WT, D-1KO, D-2KO, and D-1/2KO mice received dextran sulfate sodium (DSS) for 7 days. WT n = 13, D-1KO n = 15, D-2KO n = 14, D-1/2KO n = 15. B Experimental design for the administration of DSS. C Weight of DSS-exposed mice. D Disease activity index (DAI) of DSS-exposed mice. E Length of the colons of mice treated with DSS. F Representative H&E-stained images of proximal colon cross sections on day 12 after initial DSS exposure (left panel) and histological scores on day 12 (right panel). G Intestinal inflammation, expressed as the lipocalin levels in feces at day 0, day 7, and day 12. H Intestinal cytokines in the colon (qPCR). I Antimicrobial peptides expressed in the colon (qPCR). For statistical comparisons, (*) indicates D-1/2KO versus WT, and ($) indicates D-2KO versus WT. *p < 0.05, **^,$$p < 0.01, ***^,$$$p < 0.001