Phylogeny-guided microbiome OTU-specific association test (POST)

Microbiome

Table 3 OTUs significantly associated with bacterial vaginosis (BV) at FDR level of 0.05. TF, TreeFDR; SO, Single-OTU test implemented by POST with c = 0; DE, DESeq2; AB, ANCOM-BC; LD, LinDA; WR-P, Wilcoxon rank-sum test using proportional data; WR-R, Wilcoxon rank-sum test using the CLR transformed data

OTU	FDR-adjusted p-value								Detected method	Genus/species	Direction**
	POST	TF	SO	DE	AB	LD	WR-P*	WR-R*
OTU3	0.039	0.089	0.045	0.002	0.006	0.041	0.178	0.025	POST/SO/DE/AB/LD/WR-R	Lactobacillus crispatus	-
OTU90	0.039	0.245	0.953	0.950	0.983	0.961	0.375	0.989	POST	Lactobacillus sp.	+
OTU7	0.039	0.653	0.881	0.147	0.766	0.729	0.760	0.932	POST	Lactobacillus jensenii	-
OTU82	0.039	0.517	0.985	0.970	0.963	0.976	0.258	0.989	POST	Lactobacillus gasseri	-
OTU66	0.039	0.544	0.701	0.027	0.379	0.615	0.378	0.932	POST/DE	Lactobacillus sp.	-
OTU2	0.039	0.180	0.917	0.876	0.766	0.923	0.235	0.989	POST	Lactobacillus iners	+
OTU58	0.039	0.839	0.917	0.876	0.871	0.923	0.791	0.989	POST	Lactobacillus sp.	+
OTU112	0.411	0.046	0.701	0.422	0.379	0.678	0.040	0.932	TF/WR-P	Peptoniphilus sp.	+
OTU85	0.470	0.046	0.906	0.372	0.766	0.870	0.097	0.989	TF	Gemella sp.	+
OTU11	0.918	0.286	0.943	0.000	0.869	0.923	0.220	0.989	DE	Prevotella sp.	+
OTU12	0.391	0.089	0.701	0.013	0.338	0.615	0.040	0.942	DE/WR-P	Prevotella sp.	+
OTU16	0.680	0.155	0.881	0.001	0.766	0.918	0.097	0.989	DE	Prevotella timonensis	+
OTU91	0.680	0.092	0.881	0.505	0.766	0.852	0.040	0.989	WR-P	Prevotella sp.	+

*WR-P and WR-R did not adjust for race
**+ (and −) indicates that the OTU is positively (and negatively) associated with BV risk from a logistic regression

ISSN: 2049-2618