Fig. 7

Lactobacillus murinus may alleviate organoids H/R injury by promoting IL-10 release from macrophages via TLR2 signaling. a The proportion of IL-10⁺M2 macrophages/total M2 macrophages were analyzed by flow cytometry in the co-culture system of WT-Org and WT-MΦ or WT-Org and TLR2^−/−-MΦ or WT-Org and IL-10^−/−-MΦ. Representative quantification on the right (n = 3–4). b HE and ZO-1 and occludin immunofluorescent staining in the organoids. Scale bar = 20 μm. c Relative LDH levels were detected in organoids. d, e Relative ZO-1 and occludin fluorescence intensity and mRNA levels of ZO-1 and occludin in the organoids. The results are expressed as the mean ± SEM. n = 5–6. ***p < 0.001, **p < 0.01, *p < 0.05 were determined by one-way ANOVA (Tukey’s test). ANOVA, analysis of variance; H/R, hypoxia/reoxygenation; IL-10, interleukin-10; LDH, lactate dehydrogenase; L. murinus, Lactobacillus murinus; TLR2, toll-like receptor 2. WT-Org, organoid extracted from WT mice; WT-MΦ, macrophages extracted from WT mice; TLR2^−/−-MΦ, macrophages extracted from TLR2^−/− mice; IL-10^−/−-MΦ, macrophages extracted from IL-10^/− mice