Fig. 6

Lactobacillus murinus through TLR2 to reduce organoids H/R injury. a, b Macrophages were analyzed by flow cytometry for F4/80⁺CD206⁺CD11C⁻ cells and the expression of TLR2 on the surface M2 macrophages in the co-culture system of WT-Org and WT-MΦ or WT-Org and TLR2^−/−-MΦ. Representative quantification on the right (n = 3–4). c Relative mRNA level of Myd88 in macrophages. d HE and ZO-1 and occludin immunofluorescent staining in the organoids. Scale bar = 20 μm. e Relative LDH levels were detected in organoids. f, g Relative ZO-1 and occludin fluorescence intensity and mRNA levels of ZO-1 and occludin in the organoids. The results are expressed as the mean ± SEM. n = 5–6. ***p < 0.001, **p < 0.01, *p < 0.05 were determined by one-way ANOVA (Tukey’s test). ANOVA, analysis of variance; H/R, hypoxia/reoxygenation; LDH, lactate dehydrogenase; L. murinus, Lactobacillus murinus; Myd88, myeloid differentiation factor 88; TLR2, toll-like receptor 2. WT-Org, organoid extracted from WT mice; WT-MΦ, macrophages extracted from WT mice; TLR2^−/−-MΦ, macrophages extracted from TLR2^−/− mice