Fig. 1

Characterization of tissue injury and the gut microbiota in Sen and Res mice. Six-to eight-week-old male C57BL/6 mice underwent intestinal ischemia for 60 min, and the survival rate was observed after reperfusion. We defined mice that died within 1 h after reperfusion as Sen mice, and those that survived for 5 days as Res mice. a Changes in survival rate (n = 70) have been shown. b, c HE staining of the ileum, liver, lung, and kidney and the pathology scores. Scale bar = 100 μm. d The mRNA levels of tight junction proteins in the ileum. e Relative plasma endotoxin level. f Relative bacterial abundance at the phyla level in the feces of mice. g Alpha diversity indices. h PCoA based on the weighted UniFrac analysis of operational taxonomic units (OTUs) and anosim analysis. i Histogram of the LDA score showing the enriched bacteria in the gut microbiome of the Res mice. j Relative abundance of L. murinus at the species level in the Sen and Res mice. k Correlation between the mRNA levels of inflammatory factors in the ileum, liver, lung, and kidney and the abundance of L. murinus in the Sen and Res mice. Results are expressed as the mean ± SEM. n = 7. ***p < 0.001, **p < 0.01, *p < 0.05 were determined by two-tailed Student’s t test in c–g, adonis analysis and anosim analysis in h, Spearman analysis in k. LDA, linear discriminant analysis; L. murinus, Lactobacillus murinus; OTUs, operational taxonomic units; PCoA, principal coordinates analysis; Sen, sensitive; Res, resistant