Fig. 5

Composition of metabolic putative classes across feeding types and differentiation of known compounds. A–D Principal Coordinate Analysis (PCoA) of metabolites across all three feeding types based on 741 of the 9,723 metabolites, which is occurring in more than 50 of the 60 samples. Opacity of nodes is related to relative abundance of Mycoplasma. A All classes of metabolites, B Amino acids, peptides, and analogues, C Terpenoids, including monoterpenoid, diterpenoids, triterpenoids, and sesquiterpenoids, D Bile acids, alcohols, and derivatives. Grouping of rainbow trout reared on different feeding types were visualised as orange for CTRL, blue for PRO, and red for SYN. E Volcano plot of a differential intensity test performed between the CTRL feed against the two feed types with biotics additives. The test was based on 240 VSN normalised metabolites with a spectral match to known compounds. Metabolites with an adjusted p-value below 0.05 were significant. Size of nodes is dependent on adjusted p-value, where big nodes are significantly different between feeding types (p.adj < 0.05) and small nodes are not significant (p.adj > 0.05). The colour of nodes are dependent on related metabolic subsystems, which are specified in the legend