Fig. 3

ARG mobility potential. a Assignment of unique ARGs to plasmids, chromosomes, or both plasmid and chromosomes across samples varied by ARG classes. Green and maroon circles indicate positive and negative associations, respectively, with larger circles indicating larger correlation residuals. Only classes with more than two unique ARGs were included in the analysis. Percent abundance of ARGs b corresponding to different genetic locations; c co-located with integrative, transposable, or conjugative element hallmark genes (including transposase, integrase, and/or recombinase genes); or d co-located with non-plasmid MGEs, grouped by assigned genetic location on plasmids, chromosomes, or unclassified; e Schematic summary of the mobility potential change from influent to AS. While bar charts in panel b included unclassified reads (grey bars), the noted percentages (28%, 72%, etc.) were calculated only among classified reads in a sample, after excluding unclassified reads