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Fig. 3 | Microbiome

Fig. 3

From: Accelerated discovery of novel glycoside hydrolases using targeted functional profiling and selective pressure on the rumen microbiome

Fig. 3

Targeted functional profiling of rumen xylanases and endoglucanases, and theoretical 3D structure of xylanase 1. A Heatmap showing the distribution of the most abundant members of the GH11 and GH45 families quantified according to ShortBRED [17]. B Phylogenetic tree of xylanases and endoglucanases detected in the rumen metatranscriptome generated by the neighbor-joining method. C Theoretical 3D structure of xylanase 1 showing the labeled residues involved in the binding of 1,2-Deoxy-2-Fluoro-Xylopyranose (DFX) (PDB ID: 1c5iA), as calculated by I-TASSER [31]. D Electrostatic surface analysis of xylanase 1 generated by Chimera [34], with a color spectrum that varies from electronegative (red) to electropositive (blue) values. The ligand DFX is bound to the enzyme at the putative active site groove with electronegative residues

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Microbiome

ISSN: 2049-2618

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