Fig. 1

Broad-spectrum antibiotic treatment prevents H. diminuta-evoked inhibition of colitis. Male BALB/c mice were treated as shown in panel A (H. dim, H. diminuta 5 cysticercoids orally; DNBS, 3 mg ir.; ABX-drinking water ad libitum), and 3 days after DNBS, disease severity was assessed by B change in body weight, C colon length, D disease activity score, and E histopathology score (representative H&E images in suppl. Fig. 1). Panel F shows il-10 production by conA-stimulated splenocytes (2 μg/mL, 5 × 10⁶ cell/mL, 48 h) (data are mean ± SEM combined data from 3 to 4 experiments (data in panel F are from 2 experiments); ABX, antibiotic cocktail of kanamycin (40 mg/L), gentamicin (3.5 mg/L), colistin (4.2 mg/L), and metronidazole (21.5 mg/L); vancomycin (Van), 200 μL of 0.5 mg/mL by intraperitoneal injection; *, #,† p < 0.05 compared to control, DNBS and DNBS+ABX, respectively; pi, post-infection)