Fig. 1
From: Increasing the power of interpretation for soil metaproteomics data
Workflows assessed for metaproteomics analysis of environmental samples. The left (green) and top (blue) arrows show the experimental steps tested to obtain MS/MS spectra and metagenomics reads. The dashed-line panel presents the bioinformatics analysis, with, at the top, database construction either from metagenomics data with or without assembly step, or from generic databases. The bottom part shows the process used to refine and enhance the attribution rate through multiple search rounds. A first analysis on full databases was used to select protein-based subsets at various p-values. New searches on these subsets then identified the databases performing best