Table 1 Paired primers for qPCR
Gene | Primer sequence (5′-3′) | |
|---|---|---|
Forward | Reverse | |
Tnf | ACGGCATGGATCTCAAAGAC | AGATAGCAAATCGGCTGACG |
Il1b | GCTACCTATGTCTTGCCCGT | GACCATTGCTGTTTCCTAGG |
Il6 | CTGCAAGAGACTTCCATCCAG | AGTGGTATAGACAGGTCTGTTGG |
COX2 | GAAGTGGGGGTTTAGGATCATC | CCTTTCACTTTCGGATAACCA |
Nos2 | CAGCTGGGCTGTACAAACCTT | CATTGGAAGTGAAGCGTTTCG |
Ocln | TTACAGGCAGAACTAGACGAC | TGATGTGCGATAATTTGCTCT |
ZO1 | TTCCCAGCTTATGAAAGGGTT | TCGCTTCTTTCAGGGCACCGTA |
Cldn1 | GCCATCTACGAGGGACTG | GAGCAGGAAAGTAGGACACC |
Cldn5 | ACTGCCTTCCTGGACCACAAC | CGCCAGCACAGATTCATACACCT |
Tlr4 | AACTTCAGTGGCTGGATT | ACTAGGTTCGTCAGATTGG |
Gapdh | ATGACTCTACCCACGGCAAG | GATCTCGCTCCTGGAAGATG |