Fig. 5

Different short-chain fatty acids profile between ob/ob and db/db mice. (a) Cecum weight (g); Cecal content weight (g); Cecal tissue weight (g). (b) Amount of acetic acid, butyric acid, and propionic acid in the cecal content (nmol/mg of dry cecal content) measured by liquid chromatography-mass spectrometry (UPLC-MS). (c) Amount of isobutyric acid, 2-methylbutyric acid, valeric acid, isovaleric acid, and hexanoic acid in the cecal content (nmol/mg of dry cecal content) measured by liquid chromatography-mass spectrometry (UPLC-MS). (d) Principal component analysis (PCA) score plot of mice based on all measured metabolic parameters. Green: CT ob lean mice, red: ob/ob mice, blue CT db lean mice, and violet: db/db mice. Data are presented as the mean ± s.e.m, *P < 0.05, ****P < 0.0001 (n = 7–10). Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test for (a–c)