Fig. 2

Survival of B. vulgatus in the presence of V. vulnificus. A and B Spot inoculation of serially diluted cocultures of B. vulgatus and V. vulnificus. Three strains of B. vulgatus, MGM001, ATCC8482, and DSM28735, were coincubated with V. vulnificus in the M9-minimal medium. The ratios of B. vulgatus to V. vulnificus in cocultures were 1:1, 1:2, and 1:5. Each mixture was incubated in a 37 °C anaerobic chamber for 6 h. To compare the survival of each species, aliquots of serially-diluted cultures were spotted on RCM agar plate for B. vulgatus (A) and LBS agar plate for V. vulnificus (B). For validation of the selective growth of two species of bacteria on each agar plate, the monocultures containing either B. vulgatus (designated as 1:0) or V. vulnificus (designated as 0:1, 0:2, and 0:5) were included. C Estimation of viable B. vulgatus cells in the presence of various concentrations of V. vulnificus. Various combinatory ratios of B. vulgatus to V. vulnificus, ranging from 1:1 to 1:10, were prepared in the M9-minimal medium and incubated in a 37 °C anaerobic chamber. After 6 h, the CFUs of B. vulgatus were enumerated by spreading aliquots of serially-diluted cultures on the RCM agar plates. The percentage of B. vulgatus CFUs in each mixture was obtained by dividing them by the number of B. vulgatus CFUs in the control (B. vulgatus only; designated by 1:0 on the x-axis)