Fig. 3

Overview of genomic characteristics of Group C Arthrobacter. a Box plot of G+C content. Group A (red); Group C (blue); boxes represent the interquartile range with horizontal lines showing maximum and minimum values, excluding outliers. Group C had significantly lower G+C content. b Scatter plot of amino acid composition. Group A (light red circles); Group C (blue circles); ***p < 0.005; *p = 0.05–0.01; ns, not significant. The composition of numerous amino acids varied significantly between Group C and Group A Arthrobacter. c Protein stability predictions calculated using SCooP. Group A (red line); Group C (blue line). The curve is for coenzyme A biosynthesis bifunctional protein, CoaC, and is representative of one of the 32 Group C proteins from a total of 86 which had reduced predicted stability (Additional file 3: Dataset S2 and Additional file 1: Fig. S6) d Box plot of amino acid bias for functional categories. Boxes represent the interquartile range of the Bray-Curtis distances; lines extending from boxes show the maximum and minimum Bray-Curtis distances; dots beyond the lines represent outliers. Biases in amino acid composition (b) were reflected in specific functional categories. e Representation of functional categories. Specific functional categories were over- or under-represented in Group C; arrows indicate relative increases (up arrow) or decreases (down arrow) in functional categories in Group C. f Representation of specific functions. Specific functional processes defined by genes or pathways were characteristic of Group C (up arrow) or had a restricted capacity in Group C (down arrow) compared to Group A (also see Fig. 4)