Fig. 4

CoHo WT and CoHo Hpa-Tg mice displayed indistinguishable acute pancreatitis phenotype. a Cohousing experiment design. WT and Hpa-Tg littermates were divided to be either housed singly (SiHo WT) or cohoused (CoHo WT and CoHo Hpa-Tg mice at 1:1 ratio) for 5 weeks. After cohousing, 16S rRNA sequencing analysis and AP induction were performed. b Pancreas (g)/body weight (g) × 1‰. c Serum amylase. d Serum lipase. e Representative images of pancreatic H&E staining. f Histologic score. g Serum TNF-α measured by ELISA. h Serum IL-6 measured by ELISA. a–h n = 10/7/7 individuals in SiHo WT/ CoHo WT/ CoHo Hpa-Tg, respectively. Data were expressed as mean ± SEM. Differences of data were assessed by ordinary one-way ANOVA or Kruskal-Wallis test depending on the sample distribution. Exact p levels were all provided. Scale bars, 500 and 200 μm, respectively. AP, acute pancreatitis; Cn, caerulein; CoHo, cohoused; Hpa-Tg, heparanase-transgenic; rRNA, ribosomal RNA; SiHo, housed singly; WT, wild type