Fig. 8

Identification of feed efficiency-associated microbiota. a Displayed from the outer to the inner circle are the sample type, Wilcoxon rank-sum test in each microbial abundance between the highest- and lowest-RFI chickens, ANOVA of RFI between the two groups with the highest and lowest microbial abundance, and association analysis using quantitative model and binary models, respectively. The adjusted P values for the significance test and association analysis are plotted as –log₂(P). The dashed blue line represents P = 0.05. Each point represents a microbial genus, and the red point means that the genus passed the significance threshold. The dashed gray line indicates that the adjusted P values from the Wilcoxon rank-sum test, ANOVA, and any one of the association analyses are < 0.05. b Number of microbial genera associated with RFI detected by each test or association analysis and their overlaps. c Pearson’s (upper diagonal) and Spearman’s (lower diagonal) correlations among the shared 8 microbial genera. Significant r values are filled in numerically. d–j Detection rates of the RFI-related microbiota in the five sampling sites