When your host shuts down: larval diapause impacts host-microbiome interactions in Nasonia vitripennis

Microbiome

Table 1 Differentially expressed immunity genes in diapausing larvae compared to non-diapausing larvae. Downregulated genes are highlighted in bold

Gene	Locus^a	Early diapause		1 month		6 months
Gene	Locus^a	Log₂ fold change	Adj.^b p value	Log₂ fold change	Adj.^b p value	Log₂ fold change	Adj.^b p value
Antimicrobial peptides
Defensin 1-1	LOC100115742			−2.91	0.016	−10.24	1.54E−15
Defensin 1-2	LOC100310843			−4.27	0.00016	−8.20	4.67E−12
Nabaecin 1	LOC100117200					−12.73	0.0012
Nabaecin 2	LOC100422736					−10.06	0.0018
Nabaecin 3	LOC100678093			−5.48	0.0092	−8.88	0.0001
Nahymenoptaecin 1	LOC100122694			−5.79	0.00062	−6.77	3.52E−05
Nahymenoptaecin 2	LOC100116503			−4.73	6.86E−05	−8.52	6.72E−12
Nasonin 1	LOC100120544					−9.97	0.00022
Nasonin 2	LOC100462678	5.74	0.0014			−8.11	2.53E−05
Nasonin 3	LOC100123729	4.55	0.028			−8.35	2.34E−05
Nasonin 6	LOC100114068			2.32	0.0091	2.22	0.0082
Phenoloxidases
ProPO	LOC100122972	4.56	1.87E−06	1.83	0.019
ProPO	LOC100116823	3.21	0.00087			−5.66	1.91E−08
ProPO	LOC100121103			−4.28	1.24E−06	−6.04	4.33E−09
GNBPs
GNBP 1-2	LOC100123967	1.91	0.0035			−1.97	0.0012
GNBP 2	LOC100313520	6.16	7.24E−15	−3.60	0.0005	−3.61	0.00033
PGRPs
PGRP	LOC100117008	1.93	0.026
PGRP	LOC100117452	3.12	6.57E−06
PGRP	LOC100119736	2.19	0.0029
PGRP	LOC100119764	2.75	0.023
PGRP	LOC100119797	2.05	0.048	2.40	0.0046	2.33	0.0039
PGRP	LOC100121589					−3.51	0.021
PGRP	LOC100121609			−3.61	0.0032	−3.99	0.0005

^aLocus tags are based on the N. vitripennis genome assembly 2.1 (Accession No. GCA_000002325.2)
^bp values were FDR-corrected using the Benjamini-Hochberg algorithm

ISSN: 2049-2618