Skip to main content

Table 1 Experimental setting

From: Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individuals

Sample Sample group Sample collection Sample handling Sample input DNA extraction
Human OB, n= 19
(n= 9 PD, n= 10 HC)
Non- sterile Sterile Brain tissue Proteinase K
GFI, n= 20
(n= 10 PD, n= 10 HC)
Sterile
PN, n= 8
(n= 6 PD, n= 2 HC)
Non-sterile
Cortex, n= 3
(SC)
Sterile Sterile
Murine SPF, n=5
GRF, n=3
Non- sterile Sterile Brain tissue Proteinase K
Sterile
Controls DNA extraction blank control (KitUKB), n= 3 NA Sterile Empty Proteinase K
Negative control (BF), n= 4 NA Sterile Tris-HCl buffer Proteinase K
Negative control (SW), n= 3 NA Sterile Sterile water Proteinase K
Positive DNA extraction control (mock community), n= 6 NA Sterile Serially diluted mock community Proteinase K
No-template control (KitQIB), n= 8 NA Sterile PCR reagents No
  1. OB olfactory bulb, GFI frontal inferior cortex, PN putamen, SPF specific pathogen free, GRF germ free, BF Tris-HCl buffer, SW sterile/autoclaved water, KitUKB kitome UKB, empty tube, KitQIB kitome QIB, PCR reagents, NA not applicable