Fig. 3

Phylogenetic and functional comparison of AS MAGs and non-AS MAGs. a A whole-genome maximum likelihood phylogenetic tree consisting of AS MAGs and non-AS MAGs. One thousand MAGs randomly selected from AS bacterial MAGs and 1000 MAGs randomly selected from other environments (Parks et al. [20]) were used to build this whole-genome tree with the same methods as in Fig. 1b. The outside percent value refers to the relative abundance of AS MAGs in each clade. b Clustering of the AS and non-AS MAGs based on the COG presence/absence matrix with the t-SNE algorithm. The 2000 MAGs in a were used to generate this figure. The points representing MAGs are colored according to the taxonomy of each MAG. c The same clustering plot as in b, with the red points representing AS MAGs and blue points representing non-AS MAGs