Table 1 Features used in Virsorter2, VirSorter, and MARVEL. Detailed explanation of each feature is provided in the “Methods” section. In VirSorter, features 3 to 6 are summed up as one feature (% of Pfam affiliated genes)

1. Hallmark gene count

x

x

2. % of viral genes

x

x

x

3. % of archaeal genes

x

x

4. % of bacterial genes

x

5. % of eukaryotic genes

x

6. % of mixed genes

x

7. % of unaligned genes

x

x

8. Average gene size

x

x

x

9. Gene overlapping frequency

x

10. Gene density

x

x

x

11. Strand switching frequency

x

x

x

12. % of ATG as starting codon

x

x

13. % of GTG as starting codon

x

14. % of TTG as starting codon

x

15. Mean of GC content

x

16. SD of GC content

x

17–27. % of RBS motifs

x