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Table 1 Checklist of minimum metadata to be collected for mosquito microbiome samples and all controls (positive and negative) processed, along with examples following existing standards of recording and reporting arthropod and genomics data. A ready-to-use interactive and customizable template of these metadata records is freely available here: https://mosquito-microbiome.org/resources/mmc-white-paper/

From: Considerations for mosquito microbiome research from the Mosquito Microbiome Consortium

Metadata fields Example
General
 Study type Field, semi-field, or laboratory
 Sample name Anopheles gambiae midgut, no template extraction/PCR control (negative control), ZymoBIOMICS microbial community standard or other known mock community (positive control), etc.
 Sample ID An_Gambiae_MG_01
 Number per sample Individual, pool of 3 individuals, etc.
 Sample taxonomy Anopheles albimanus
 Developmental stage Egg, larval instar, pupa, or adult
 Sex Male, female, or both pooled
 Agea 3 days post adult eclosion, 2–5 days post eclosion etc.
 Mating status Virgin or mated/non-virgin
 Gonotrophic status Non-gravid, fully gravid, or half-gravid
 Blood fed Non-blood fed or blood fed
 Type of food providedb 10% sucrose, human blood, 1:3 yeast and TetraMin, etc.
 Tissue processed Whole mosquito, midgut, ovaries, cuticle surface, etc.
 Sample phenotype Virus/parasite infection status, insecticide resistance status, etc.
 Collection date YYYY, YYYY-MM, or YYYY-MM-DD
 Collection time hh, hh:mm, or hh:mm:ss
 Biomolecule processed DNA, RNA, protein, metabolites, etc.
 Biomolecule isolation method QIAGEN blood and tissue, phenol-chloroform, etc.
 Sequencing method 16S rRNA amplicon, whole (meta)genome, metatranscriptomic, etc.
 Sequencing platform Illumina, Oxford Nanopore, etc.
 Sequencing platform model MiSeq (Illumina), MinIon (Oxford Nanopore), etc.
 Sample storage preservative None, Ethanol, RNALater®, etc.
 Sample storage temperature − 20 °C, − 80 °C, etc.
 Sample storage duration None, 6 months, 3 years, etc.
Specific to field studies
 Collection country Nigeria
 Collection village or city Iko Esai village
 Location coordinates 00.000000, 00 00.0000, or 00° 00′ 00.0″ N 0° 00′ 00.0″ E
 Climatic/environmental data 27 °C, 82% RH
 Land cover Savanna, urban and built up, tundra, etc. See Loveland et al. [31] for more examples
 Collection method Human landing catch, mechanical aspirator, gravid trap, or larval dipping
 Collection bait CO2, cattle
Specific to laboratory studies
 Name and location of laboratory (and/or facility) The Short Lab, College of Food, Agricultural, and Environmental Sciences, The Ohio State University, Columbus, OH, USA
 Strain STECLA, KISUMU, ROCKEFELLER
 Generation F1, F6, F59, etc.
 Maintenance temperature 27 ± 2 °C
 Maintenance relative humidity 80 ± 10%
 Light-dark cycle 10-h light; 14-h dark
Specific to semi-field studies
 Name and location of semi-field facility (if widely used/known) MalariaSphere, Mbita Point Research & Training Center, International Centre of Insect Physiology and Ecology, Mbita, Kenya [32]
 Type of semi-field structure Glass house, mesh house, mesh cage, etc.
 Dimensions of semi-field structure 00.00 × 00.00 m
  1. aMay not apply to field-derived mosquitoes
  2. bNot applicable to field-derived mosquitoes