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Table 2 Overview of tested libraries: case 1 samples. For viruses enclosed in the samples, reference genomes were included in the genome set used for VirBait design. Sample matrix, animal species, the detected virus, and the references are given. The amount of virus reads in the sequence datasets found in the originally sequenced libraries and in the VirBaits treated libraries is given as percentage. Libraries were made from RNA except the one marked by an asterisk, which was made from DNA

From: Next-generation diagnostics: virus capture facilitates a sensitive viral diagnosis for epizootic and zoonotic pathogens including SARS-CoV-2

Library ID

Sample material

Verified virus

Virus portion

Data set size VirBaitsa

Reference

Generic HTS

VirBaits

L02725

Brain, Strix nebulosa

WNV

0.14%

58.7%

2678

[20]

L02726

Spleen, Strix nebulosa

WNV

5.6%

91.4%

3857

[20]

L03224

Spinal cord, Equus sp.

WNV

Not sequenced

0.2%

1,305,078

Unpublished

L03038

Brain, Bubo scandiacus

WNV

0.0003%

0.03%

155,648

Santos et al., submitted

L03451

Spinal cord, Equus sp.

WNV

0.0006%

0.2%

907,780

[21]

L02838

Brain, Canis lupus

RABV

0.002%

7.9%

158,514

Unpublished

L03007

Brain, Tragelaphus sp.

RABV

1.5%

97.5%

890,113

Unpublished

L03015

Salivary gland, Tragelaphus sp.

RABV

0.005%

45.8%

1,060,955

Unpublished

L02912

Brain, Rattus sp.

BoDV

0.75%

85.2%

183,579

Schlottau et al., in preparation

L02913

Brain, Rattus sp.

VSBV-1

0.0004%

0.6%

42,793

Schlottau et al., in preparation

L02456

Soft Palate Cells, Bos taurus

FMDV

0.002%

2.0%

719,153

[22]

L02568*

Food matrix spiked with a mock community

BHV

0.0003%

0.04%

181,338

[23]

L02569

BVDV

BDV

0%

0.00001%

0.007%

0.01%

506,465

L02762*

Spleen, Sus scrofa

ASFV

0.05%

20.7%

1,353,258

Unpublished

L02651

Organ pool, Gallus gallus

IAV (H5N8)

0.01%

70.1%

280,247

Unpublished

L03706

Brain, Gallus gallus

IAV (H3N1)

0.5%

77.0%

317,862

Unpublished

L03827

Nasal wash, Mustela putorius

SARS-CoV-2

0.3%

53.8%

1248

[24]

L03828

Nasal wash, Mustela putorius

SARS-CoV-2

0%

9.1%

449

[24]

L03829

Nasal conchae, Mustela putorius

SARS-CoV-2

0.00004%

0.001%

194,891

[24]

L03830

Nasal conchae, Mustela putorius

SARS-CoV-2

0.02%

14.9%

325,694

[24]

L03862

Nasal conchae, Mustela putorius

SARS-CoV-2

0.0004%

0.2%

960,594

[24]

L03862

Nasal conchae, Mustela putorius

SARS-CoV-2

0.001%

0.5%

946,268

[24]

L03862

Brain, Mustela putorius

SARS-CoV-2

0.00002%

0.005%

294,146

[24]

  1. adataset size is given in terms of reads