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Fig. 1 | Microbiome

Fig. 1

From: High-quality bacterial genomes of a partial-nitritation/anammox system by an iterative hybrid assembly method

Fig. 1

Iterative hybrid assembly workflow for enrichment ecosystems. Subsampled short reads (SRs) and long reads (LRs) are first prepared from the total sequenced dataset or from the simplified dataset that eliminates reads used for assembling qualified MAGs (step 1). SRs and LRs are then combined for hybrid assembly using Unicycler and then binning with MetaWRAP (step 2). Qualified MAGs with high quality and high contiguity were selected (step 3). SRs and LRs clusters for residual microbes in the community are obtained by taking out reads of qualified MAGs and then subsampling for the next run (step 4)

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