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Table 2 Summary of the ability of the hit clones to hydrolyze human glycans. Cell extracts, containing one (clone 3 and clone 5) or several GHs (clone 1, clone 2, and clone 4), were incubated with 2 mM host-derived oligosaccharides for 24 h at 37 °C in 50 mM sodium phosphate buffer pH 7, before analysis by HPAEC-PAD (detailed in Fig. S4). The tested oligosaccharides, which share structural homologies with intestinal mucins, were the aGM2, GM2, tGB4, and GM1a ganglioside sugars, and the HMOs lacto-N-triose (LNT2), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT). The E. coli Epi100 screening strain constitutes the negative control. The purified Uhgb_GH123 enzyme isolated in clone 5 was tested in the same conditions on these substrates, using 2 μM of enzyme.

From: Investigating host-microbiome interactions by droplet based microfluidics

  1. Keys: -, no hydrolysis; +, partial hydrolysis; +++, complete hydrolysis; blue circle, Glc; yellow circle, Gal; blue square, GlcNAc; yellow square, GalNAc; purple diamond, neuraminic acid