Fig. 2

Validation of cell architecture and numbers in a Protoblock. a Flow cytometry dot plots measuring the cell density of fixed bacterial suspensions used to make Protoblocks. Events were gated either for SYTOBC+ cells or beads. The averages of 3 reads for 4 populations per cell type are shown here and in Table 1. b Light microscopy images confirming cell architecture of Protoblock slides stained with H&E (4T1 cells) or Gram staining (bacteria). c Fluorescence microscopy images confirming cell content of Protoblocks. Slides were with α-E. coli (green), α-S. aureus (red) or DAPI (grey). Counts in Fig. 1 are the average of 20 FOV in 3 × 4 μm slides