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Fig. 3 | Microbiome

Fig. 3

From: Climate mediates continental scale patterns of stream microbial functional diversity

Fig. 3

Projected changes across the Eurasian river network in functional gene alpha diversity and assemblage composition under future climate scenarios. The relative increase in Shannon diversity assuming the moderate emission scenario RCP 4.5 (a, map) and the relative increase in Shannon diversity averaged by latitude for the three emission scenarios (a, line plot) were calculated by linear models using temperature of the warmest quarter (TWQ) and precipitation of the coldest quarter (PCQ) as predictors (R2 = 0.543; P < 0.001). The turnover rates assuming the moderate emission scenario RCP 4.5 (b, map) and the turnover rates averaged by latitude for the three scenarios (b, line plot) were calculated using generalized dissimilarity models with the TWQ and PCQ as predictors on Bray-Curtis dissimilarity matrices (D2 = 65.6%; P < 0.001). The violin boxplots show the median and the first and the third quartiles for the relative increase in Shannon diversity (c) and the turnover rate (e) on the overall gene pool and for the mean relative change in Shannon diversity (d) and the mean turnover rate (f) on every gene family pool, grouped into functional categories. Pairwise differences across functional categories regarding the relative change in the Shannon diversity (d) and turnover rates (f) were examined by a Bonferroni-corrected pairwise t-test (P < 0.05) post hoc analyses and are indicated with the symbols +, ▲ and . Light and dark grey areas in maps depict the climate envelope covered by and extrapolated from the in situ data, respectively, in terms of TWQ and PCQ. C, carbon; N, nitrogen; P, phosphorus; S: sulphur; St, stress

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