Construction of habitat-specific training sets to achieve species-level assignment in 16S rRNA gene datasets

Microbiome

Table 1 The eHOMD training sets performed well for species/supraspecies taxonomy assignment of an independent long-read 16S rRNA gene sinonasal dataset

	# ASVs	# Reads	% ASVs	% Reads
NCBI 16S Microbial blastn	178	140,455	87.3	97.3
eHOMD blastn	188	143,274	92.2	99.3
eHOMD FL_Compilation_TS	194	142,843	95.1	99.0
eHOMD V1V3_Supraspecies_TS	201	144,279	98.5	99.9

Columns three and four indicate the % of ASVs and reads, respectively, assigned subgenus-level taxonomy using each of the four approaches tested

ISSN: 2049-2618