Fig. 4
Gar in the six identified genetic contexts. a PETL metagenomics DNA sample. Primers HS458-HS459 amplified the region between attI site and qacEΔ of a clinical class 1 integron (GenBank accession MN215968). bLuteimonas sp. 83-4 (CP029556.1), chromosomal, not annotated in the available contig sequence. cP. aeruginosa 105MG (AJ786649.2 and VLOE00000000), chromosomal, clinical class 1 integron, included in a Tn5051-like transposon. Gar variant of P. aeruginosa 105MG = orfPa105, previously annotated as hypothetical protein. dP. aeruginosa S742_C15_BS (NFFO01000062.1), clinical class 1 integron, downstream context identical to P. aeruginosa 105MG, but lacking the Tn21-like region upstream. eS. enterica subsp. enterica serovar Johannesburg FSIS1609335 (AAIUOI010000042.1), class 1 integron, downstream flanked by Tn21 elements, ISPa38 and IS110. f S. enterica ADRDL-2811 (AAKHBQ010000151.1), downstream flanked by Tn21 elements. a, d–f Entire contig shown. Attachment sites: attI, marked as blue rhomb; attC, marked as green rectangle; hp, hypothetical protein; aspA, aspartate ammonia lyase; catB3, chloramphenicol transferase; aph(3′)-XV, aminoglycoside phosphotransferase; xerC, tyrosine recombinase (integrase); IS1071 (Tn3 family): single element, including transposase and flanking inverted repeats; Tn5051-like tnp region consisting of tnpA: transposase, tnpR: resolvase and tnpM: putative transposition regulator; intI1, class 1 integron integrase; blaVIM-1, metallo-β-lactamase VIM-1; qacEΔ, quarternary ammonium compound resistance protein (truncated); GNAT, Gcn5-related N-acetyltransferase; tniA–tniR and urf-2, tni region (Tn21-like); aac(6′)-Ib, aminoglycoside acetyltransferase; blaOXA-2, class D β-lactamase OXA-2; ant(3″)-Ia, aminoglycoside nucleotidyltransferase; ISPa38 (Tn3 family); and an IS110 family element