Entomopathogenic nematode-associated microbiota: from monoxenic paradigm to pathobiome

Microbiome

Table 1 Lethal time 50 values (LT₅₀) for Spodoptera littoralis following the injection of bacterial strains into the haemocoel of last-instar larvae (incubated at 23 °C)

	Strain	Dose range	LT50 (h)^a
S. carpocapsae microbiota	Xenorhabdus nematophila XnSc_F1	10²–10³	27
	Pseudomonas protegens PpSc_PP-SC-10	10²–10³	29
	Stenotrophomonas maltophilia StmSc_ALL5	10⁵–10⁶	No mortality
	Alcaligenes faecalis AlcfSc_SC	10⁵–10⁶	No mortality
	Pseudochrobactrum PochSc_AL3	10⁵–10⁶	No mortality
	Ochrobactrum sp OchSc_ALL4	10⁵–10⁶	No mortality
S. glaseri SK39 microbiota	Xenorhabdus poinarii XpSg_G6	10²–10³	No mortality
	Pseudomonas protegens PpSg_SG6 Apo	10²–10³	28
	Pseudomonas chlororaphis PcSg_SK39 ApoA	10²–10³	19
	Stenotrophomonas maltophilia StmSg_SK39-2	10⁵–10⁶	No mortality
S. weiseri 583 microbiota	Xenorhabdus bovienii XbSw_CS03	10⁵–10⁶	No mortality
	Pseudomonas protegens PpSw_SW4	10²–10³	20
	Pseudomonas protegens PpSw_TCH07 2-2	10²–10³	26
	Stenotrophomonas maltophilia StmSw_SW1	10⁵–10⁶	No mortality
	Stenotrophomonas maltophilia StmSw_TCH07 2-3	10⁵–10⁶	No mortality
	Ochrobactrum anthropi OchaSw_SW2	10⁵–10⁶	No mortality
H. bacteriophora microbiota	Photorhabdus luminescens laumondii PholHb_TT01	10³	32
H. bacteriophora microbiota	Ochrobactrum anthropi OchaHb_B3	10⁵–10⁶	No mortality
Other Pseudomonas strains	Pseudomonas protegens CHAO^T	10²–10³	28
Other Pseudomonas strains	Pseudomonas chlororaphis CFBP 2132^T	10²–10³	19
Negative control for pathogenicity	Escherichia coli CIP 7624	10⁵–10⁶	No mortality

^aMortality was recorded over 72 h following the intrahaemocoel injection of 10²–10³ or 10⁵–10⁶ washed bacterial cells/larva. LT₅₀ was calculated on 20 larvae

ISSN: 2049-2618