Bacterial communities associated with IJs of S. carpocapsae SK27, Galleria mellonella larvae and control samples. The IJ microbiota was investigated by metabarcoding with the V3V4 region of the 16S rRNA gene. a Venn diagram of the OTU detected in of S. carpocapsae SK27 batch SK27_23_08_16 samples, G. mellonella samples and control samples (Kitome_QE, Kitome_MN, Tap water and Ringer). OTUs (frequency cut-off > 80% and abundance cut-off > 0.01%) were assigned to genus level. Non-core set embeds OTUs that are not shared by all the control, the Galleria or the Steinernema replicates. b Heatmap showing the composition of the microbiota of S. carpocapsae SK27 batch SK27_23_08_16 samples. Each column represents a technical replicate. The 30 most abundant OTUs across the samples at the genus level of affiliation (Top30 Genus) are listed on the left. The percentage relative abundance is indicated by the gradient of blue hues.