Comparison of the compositions of fecal indicators, ARGs, OTUs, and functional genes between upstream and downstream. a Relative abundances of human fecal indicators compared to that of ARGs. The abundance of 27 representative human fecal OTUs was estimated based on the proportions of 16S rRNA gene amplicon reads. The abundance of crAssphage was estimated based on the depth of aligned metagenome reads and normalized by the average read depth of SCGs. b Correlation between abundance of ARG and abundance of human fecal indicators. c β-Diversity of the compositions of ARGs, OTUs, and functional genes of Han River samples. Plot coordinates were determined by principal coordinate analysis of Bray–Curtis dissimilarity matrix, based on the ARG compositions analyzed from metagenome reads (left), OTU compositions from 16S rRNA gene amplicon data (middle), and COG compositions analyzed from metagenome contigs (right).