Fig. 7

Marker genes identification and correlation analysis. a Heatmap of detected marker genes (cox1, gyrB, and recA) in NGS data of Aedes aegypti and Culex quinquefasciatus pools. The color of the heat map indicates the RPKM of the genes. The Aedes aegypti and Culex quinquefasciatus pools are highlighted with orange and green background, respectively. Pools containing five mosquitoes are marked with red dots and the sample marked with a star is the special sample Ab-AAF-1-3. b Correlation analysis on relative abundance of confirmed phage contigs (> 1500 bp), bacteria, and mosquito marker genes. The gradation of red color in the circle indicates the degree of positive correlation. The bigger size of the circle associates with lower p value. Only the correlations with an adjusted p value less than 0.01 are shown in the figure. The marker genes are labeled with red font color and phage contigs are labeled with black font color. Phage contigs of which WIsH predicted the genus Wolbachia as the host (p < 0.001) are marked in gray