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Fig. 1 | Microbiome

Fig. 1

From: A new family of uncultivated bacteria involved in methanogenesis from the ubiquitous osmolyte glycine betaine in coastal saltmarsh sediments

Fig. 1

A novel clostridial bacterium involved in glycine betaine (GBT)-dependent methanogenesis from a salt marsh sediment. a Microcosm incubations of salt marsh sediments with the addition of GBT. Methane formation and GBT and trimethylamine (TMA) concentrations in the sediment slurry were measured. Microcosms were set up in three biological replicates, and error bars represent standard deviations. Samples were taken from the microcosms at T0, T1, T2, and T3 for amplicon sequencing analyses. b A working model of GBT degradation by bacterial degraders which produce TMA and a two-carbon compound, and archeal methanogens which produce methane from TMA. The red dots highlight the carbon atoms in GBT which are labelled with 13C. c Miseq amplicon sequencing of microbial 16S rRNA genes during GBT-dependent methanogenesis. d Increase of relative abundance of this novel clostridial bacterium over time in the GBT-amended microcosms

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