Fig. 3

Phylogeny of 120 concatenated proteins from 121 bacterial genomes, including the 58 MAGs from the present study. The identification and alignment of the 120 conserved proteins were performed using the GTDB-Tk [53]. The MAGs recovered from this study are in white. The completeness of these MAGs was estimated using CheckM [40]. MAGs that dominate in the studied PNA reactor (long-term SBR operation) and seed sludge (continuous operation) are in white bold font with red and blue backgrounds, respectively. The unique MAGs that are only recovered from seed sludge are in white bold font with gray backgrounds. Rare populations that have relative abundance < 2% in the seed and long-term operated PNA reactor are in white bold font with brown background. Kartal et al. [19] reported MAGs are in green bold font