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Table 2 The deaminase activity of 1-aminocyclopropane-1-carboxylate (ACC) and the indole-3-acetic acid (IAA) concentrations in different strains of Kosakonia sp. S1 (S1), Enterobacter sp. E5 (E5), Klebsiella sp. Kb (Kb), and the engineered strains of E5, S1, and Kb (designated as E5P, S1P, and KbP, respectively) by expressing deaminase on the bacterial cells

From: Engineering banana endosphere microbiome to improve Fusarium wilt resistance in banana

 

Concentration of α-ketobutyrate (mM)

Concentration of IAA (mg L−1) by Salkowski reagent

With toluene

Without toluene

Without Trp

With Trp

Control

0.00 ± 0.00b

0.01 ± 0.00b

0.33 ± 0.04c

0.41 ± .005c

S1

0.06 ± 0.01b

0.03 ± 0.01b

27.1 ± 1.33b

39.5 ± 3.46b

S1P

4.94 ± 0.35a

2.05 ± 0.13a

ND

ND

E5

0.06 ± 0.01b

0.05 ± 0.01b

22.6 ± 2.10b

45.1 ± 2.73b

E5P

4.82 ± 0.17a

1.96 ± 0.35a

ND

ND

Kb

0.04 ± 0.01b

0.03 ± 0.01b

155 ± 11.2a

161 ± 12.9a

KbP

0.11 ± 0.01b

0.08 ± 0.01b

ND

ND

  1. The different letters within columns indicate that the values are significantly different at P < 0.05. Values are reported as mean ± SD (standard deviation) (n = 5)
  2. ND not determined