Fig. 4

Abundance of methanogenesis pathways as well as of archaeal and bacterial acetyl-CoA decarbonylase/synthase (ACDS). Spectral counts of representative metaproteins for A.) methanogenesis pathway and B.) each ACDS metaprotein (Additional file 5: Table S3 E_Methanogenese) sorted by archaeal and non-archaeal and summed. The black bars indicate bacterial one carbon metabolism and hydrogenotrophic methanogenesis. The red bars are associated with either acetoclastic methanogenesis or acetoclastic methanogenesis as well as the methanol and methylamine pathways. Differences between both groups of BGPs were validated by student’s t-test and highlighted by “*” and the associated p-values. The parentheses under the sample names on the x-axis show the total number of identified microbial spectra for each BGP