Fig. 3

Distribution of enzymes involved in metabolic pathways in culture-independent and culture-enriched metagenomes. Enzymes were annotated in the metagenomic assemblies of CIM and CEM samples based on Enzyme Commission groups, and their taxonomical origin was inferred based on contig sequences. a The distribution of the taxonomical origin of enzymes at the genus rank. b The distribution of enzymes in the CIM and CEM samples. In both cases, black bars indicate the presence of an enzyme-associated genus or sample. Panels A and B are meant to be interpreted together as they share the same horizontal axis, which represents individual enzyme groups. Colors in a are clusters of EC numbers based on their presence or absence in CIM and CEM. c Coverage of pathways in the 12 clusters defined in a and b. The coverage represents the percentage of genes from the pathway that was observed in a cluster. Colored bars on the left of c are pathway categories