Fig. 1
Diversity and ecology of family S24-7 species. In all figure panels, only samples with S24-7 matches ≥ 0.25% relative abundance were considered for plotting, a threshold of confidence below which the risk of including spurious OTUs in the analysis increases substantially. a Cladogram of the family’s diversity (based on a Neighbor-Joining tree) as supported by existing full-length 16S rRNA gene sequences (n = 7784 after quality checks). The colored ring (color code corresponds to panel b) indicates the origin of samples recorded in IMNGS-derived samples (www.imngs.org) with the highest relative abundance for the given molecular species. The outer black bars represent the values of these maximum relative abundances (see scale). Terminal tree branches colored in red indicate species with existing genomic information, corresponding to reconstructed metagenomic species, short-term isolates, or those able to be maintained in culture and taxonomically described in this study (yellow stars). b Summary of sample origins recorded in IMNGS with the highest relative abundance of sequences for the 685 molecular species shown in panel a. The category “unknown” corresponds to S24-7 species for which the full-length 16S rRNA gene sequence returned no match to any of the amplicon sequences in existing IMNGS samples at > 97% sequence similarity over > 90% sequence length. c Distribution of highest relative abundances across all molecular species. Only positive species were considered for plotting, i.e., species of unknown origin (no sequences detected in amplicon datasets) or occurring at < 0.25% relative abundance were ignored. d Prevalence of S24-7 spp. in samples of different origins. Each bar represents the percentage of samples in the given category that were positive for S24-7 species. The column labeled “#inDB” provides the total number of samples per category. e Number of co-occurring S24-7 species in the tested human and mouse gut samples and distribution of the cumulative relative abundance of the family in the mouse gut (positive samples only)