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Fig. 4 | Microbiome

Fig. 4

From: Restriction enzyme digestion of host DNA enhances universal detection of parasitic pathogens in blood via targeted amplicon deep sequencing

Fig. 4

Enzyme digestion markedly lowers assay limit of detection. (a) Reads per thousand for undigested (gray) and digested (black) 10-fold serial dilutions of P. knowlesi in whole human blood (n = 4, mean ± SD). (b) Log-transformation of reads per thousand from serially diluted samples suggests a limit of detection of 163 parasites per microliter for undigested samples (gray, r2 = 0.9852) and 15 parasites per microliter for digested samples (black, r2 = 0.9533) (n = 4 biological replicates, mean ± SD). (c) After deeper analysis, reads per thousand for undigested (gray) and digested (black) serial dilutions between 61 parasites per microliter and 0.72 parasites per microliter demonstrate a limit of detection of 40 to 60 parasites per microliter for undigested samples and 7 to 29 parasites per microliter for digested samples (two-way ANOVA with Sidak’s multiple comparisons posttest, **** p < 0.0001, *** p < 0.001, ** p < 0.005 n = 3, mean ± SD)

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Microbiome

ISSN: 2049-2618

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