Fig. 3

MGSs analysis. a The heatmap of 25 ‘tracer’ genes abundance for each MGS were shown. Individuals are represented along the horizontal axis. Abundance of genes in rows is indicated by color gradient (white, not detected), and the enrichment significance is shown on the right with P value by Wilcoxon test. b The relative importance of each MGS in the predictive random forest model using the mean decreasing accuracy. c Relationship between the numbers of MGSs included in random forest model and the corresponding predictive performance (estimated by 10-fold cross-validation). d The ROC curve for the random forest model using 13 MGSs